First Report of Barley virus G in the United States and California

HomePlant DiseaseVol. 105, No. 10First Report of Barley virus G in the United States and California PreviousNext DISEASE NOTE OPENOpen Access licenseFirst CaliforniaA. Erickson B. W. FalkA. EricksonDepartment Plant Pathology, University California, Davis, CA 95616Search for more papers by this author Falk†Corresponding author: Falk; E-mail Address: [email protected]://orcid.org/0000-0002-5431-1627Department AffiliationsAuthors Affiliations A. Falk † Department 95616 Published Online:26 Oct 2021https://doi.org/10.1094/PDIS-03-21-0478-PDNAboutSectionsView articlePDFPDF Plus ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleBarley (Hordeum vulgare) is a valuable annual cereal crop grown widely throughout world. The majority barley commercially used livestock feed, with remainder being malting industry and, lesser extent, direct food consumption; it also often employed as cover (Lazicki et al. 2016). Yellow dwarf viruses (YDVs), family Luteoviridae, which infect other crops, are common distributed (Griesbach 1989; Seabloom 2009). In April 2018, five samples exhibiting typical symptoms YDV infection (primarily yellowing leaf margins tips) collected from fields Yolo county planted cultivar Butta 12 were tested viruses. Total RNA was extracted tissue using TRIzol reagent, according manufacturer’s protocol. template multiplexed RT-PCR assay designed generic detection infecting YDVs, protocol established Malmstrom Shu (2004). A 372 base pair amplicon indicative polerovirus amplified two sequenced (Quintara Biosciences), resulting data analyzed via BLASTn search. No further testing or work done three that negative. Not unexpectedly, top result returned one positive yellow virus-RPV (CYDV-RPV; 98% identity), cereals States. Unexpectedly, however, sample (BVG), sharing 98.43% identity Uiseong BVG isolate (GenBank accession LC259081). To confirm presence sample, full-length viral genome two-step primers targeting extreme 5′ 3′ ends genome, PrimeScript RT PrimeSTAR GXL DNA Polymerase kits (Takara Bio), cloned into binary vector pJL89, BLAST search 5,621 nucleotide sequence (100% query coverage) once again results showing be BVG. deposited GenBank (MW853785). Nucleotide comparisons showed shares 96.62, 96.57, 96.02% sequences BVG-Gimje (KT962089), BVG-Uiseong (LC259081), BVG-Aus8 (LC500836) isolates, respectively. our knowledge, first report Currently prevalence, host range, mode timing introduction unknown; its impact on production yield any location has been identified thus far unknown may warrant investigation.The author(s) declare no conflict interest.References:Griesbach, J. A., 1989. Calif. Agric. 43:23. Google ScholarLazicki, P., 2016. https://apps1.cdfa.ca.gov/FertilizerResearch/docs/Barley_Production_CA.pdf. ScholarMalmstrom, C. M., Shu, R. 2004. Virol. Methods 120:69. https://doi.org/10.1016/j.jviromet.2004.04.005 Crossref, ISI, ScholarSeabloom, E. W., 2009. Am. Nat. 173:E79. https://doi.org/10.1086/596529 ScholarThe interest.DetailsFiguresLiterature CitedRelated Vol. 10 October 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPycnidia Leptosphaeria biglobosa formed necrotic blighted petiole Brassica rapa subsp. pekinensis (C. Yu al.). Photo credit: Yu. Panicle blast rice site sub-Saharan Africa (S. K. Mutiga V. Were. Symptoms bud soft rot agave caused Pantoea dispersa (F. Palemón-Alberto S. Ortega-Acosta. Metrics Downloaded 472 times Article History Issue Date: 28 Dec 2021Published: 26 2021First Look: 27 Apr 2021Accepted: 15 2021 Page: 3312 Information© American Phytopathological SocietyKeywordspolerovirusLuteoviridaebarleyThe interest.